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After 30 min incubation at room temperature, the treated lysates were further divided into smaller aliquots (50 l), which were then heated at different temperatures for 3 min on a Veriti thermal cycler (Applied Biosystems) followed by cooling for 3 min to room temperature

These cytokines contribute to the recruitment and activation of other immune cells, such as neutrophils, and promote the production of additional inflammatory mediators (Zong et al., 2025)

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